Macrophage migration inhibitory factor researchgate. Mif 60 nm was incubated with test compounds for 5 min at room temperature. Recently, mif was reevaluated as a proinflammatory cytokine and pituitaryderived hormone that. Macrophage migration inhibitory factor mif interferes with. Mif enhances the secretion of inflammatory mediators, and there is a strong association between mif and colitis.
For human studies, the production of migration inhibitory factor can be assayed using the indirect method. Macrophage migration inhibitory factor contributes to the. Mif is an acidic protein with a mol wt of approximately equal to 25,000 daltons as determined by gel filtration and analytical polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Migration inhibition factor definition of migration. Macrophage migration inhibitory factor is a molecular. Thirtyyears ago it was described as a tcellderived factor that inhibited the random migration of macrophages in vitro. As the mif function in congenital toxoplasmosis is not fully elucidated yet, the present study brings new insights for t.
Journal of clinical medicine article myocardial expression of macrophage migration inhibitory factor in patients with heart failure julia pohl 1, id, ulrike b. Macrophage migration inhibitory factor is subjected to. Despite the growing numbers of people suffering from blel, the etiology and mechanisms underlying its pathogenesis remain unknown. After repeating this procedure on remaining intestinal pieces for three. Although first described as a t cellderived activity, mif has been shown recently to be released by a variety of cell types. Expressions of macrophage migration inhibitory factor in. A lymphokine which inhibits the migration of macrophages away from the site of interaction between lymphocytes and antigenss. Upon releases mif modulates the expression of several inflammatory molecules, such as tnf, nitric oxide and cyclooxygenase 2 cox2. The p values were calculated by students t test, p.
Macrophage migration inhibitory factor mif makes chemokinelike functions and plays critical roles in various inflammatory diseases. Patients undergoing openheart surgery are susceptible to complications, including acute kidney injury aki. The human macrophage migration inhibitory factor mif gene is composed of three short exons green boxes of 107, 172 and 66 base pairs, and two introns pink boxes of 188 and 94 base pairs. We previously showed that mif is a potent modulator of p53 and e2fdependent pathways that are activated in response to oncogenic signaling. Therapeutic cytokines, immunotoxicological evaluation of migration inhibitory factor mif springerlink. New therapies that improve host immunity could reduce influenza virus disease burden.
Jci insight macrophage migration inhibitory factor. The appearance of macrophage migrationinhibition factor in. A direct macrophage migration test is described, which proved to be useful for the. Journal of immunological methods, 12 1976 275283 275 northholland publishing company, amsterdam printed in the netherlands an evaluation of the methods available for analysing results from migration inhibitory factor mif test j. Mif has been reported to play a central role in tumor cell proliferation and invasion in several. Jci the proinflammatory mediator macrophage migration. Macrophage migration inhibitory factor mif is a cytokine that is secreted by the anterior pituitary and immune cells in response to surgical stress, injury, and sepsis. Macrophage migration inhibitory factor mif is an important constituent of the host response to stress and infection and is the first mediator that has been identified to be released from immune. Prognostic value of serum macrophage migration inhibitory. The protective role of macrophage migration inhibitory.
Mif is a glycoprotein that presents a wide spectrum of biological activities and exerts a complex interaction with various cellular signaling pathways, causing imbalance of homeostasis. Macrophage migration inhibitory factor mif emerged in recent years as an important inflammation mediator, playing a prominent role in the pathogenesis of various types of malignant neoplasm. Today, mif is known to be a mediator of the function of macrophages in host defense and its expression. Uremic cardiomyopathy is a risk factor of endstage renal disease esrd and is responsible for high mortality rates and increased left ventricular mass index lvmi. Mif is secreted by activated t lymphocytes and macrophages upon exposure to lipopolysaccharide and induces secretion of tumor necrosis factor. Macrophage migration inhibitory factor mif promotes inflammation and is an important factor in uremic cardiomyopathy. Renal ischemiareperfusion injury induced more inflammation and kidney cell death in mice lacking mif than in wild. A direct macrophage migration inhibition test applied in man. Many cells express mif, including epithelial, endothelial, and most immune cells. Macrophage migration inhibitory factor mif was originally found to be a pituitary hormone, inflammatory cytokine, and glucocorticoid.
Macrophage migration inhibitory factor mif request pdf. Frontiers macrophage migration inhibitory factor mif. Moreover, the pivotal nature of its involvement highlights the importance of mif to the. Macrophage migration inhibitory factor mif is a cytokine that plays a central role in immune and inflammatory responses. The report provides comprehensive information on the macrophage migration inhibitory factor glycosylationinhibiting factor or ldopachrome isomerase or mif or ec 5.
Macrophage migration inhibitory factor is subjected to glucose modification and oxidation in alzheimers disease. In the present study, we used gene and cytokines expression profiling, western blot and immunohistochemistry to get further insight into the cellular and molecular. Absolute measurements of macrophage migration inhibitory. The aim of this study is to investigate the effects of serum macrophage mif on myocardial. To evaluate whether, in patients with the diffuse form of systemic sclerosis dssc, macrophage migration inhibitory factor mif production is dysregulated. However, the potential roles of mif in regulating hepatocellular carcinoma hcc tumor cell migration and the expression of angiogenic factors by hcc. The role of macrophage migration inhibitory factor in the function. Mif is a pleiotropic proinflammatory cytokine that plays a causative role in multiple diseases, including cancer. Macrophage migration inhibitory factor mif is a proinflammatory cytokine that is a critical upstream mediator of the innate immune response. Migration inhibitory factor financial definition of. Macrophage migration inhibitory factor mif functions as a pluripotent cytokine involved in broadspectrum pathophysiological events in association with inflammation and immune responses. Colitisassociated colorectal cancer crc development has been shown to be related to chronically enhanced inflammation. An evaluation of the methods available for analysing. Migration inhibitory factor mediates angiogenesis via.
Factor a financial institution that buys a firms accounts receivable and collects the accounts. For statistical analysis, test of normality and mannwhitney utest were used. Factor a third party that buys a firms accounts receivable. Macrophage migration inhibitory factor mif may contribute to multiple aspects of tumor progression, including control of cell proliferation, differentiation, cell survival and angiogenesis. Macrophage migration inhibitory factor promotes the. Pdf recombinant human migration inhibitory factor has.
Renal ischemiareperfusion injury induced more inflammation and kidney cell death in mice lacking mif than in wildtype mice. The human macrophage migration inhibitory factor mif gene is composed of three short exons green boxes of 107, 172 and 66 base pairs, and two. Here, we investigated the role of mif in the inflammatory response. Hebert central veterinary laboratory, weybridge, england received 9 march 1976. The mif protein superfamily also includes a second member with functionally related properties, the ddopachrome tautomerase ddt. Global markets directs, macrophage migration inhibitory factor glycosylationinhibiting factor or ldopachrome isomerase or mif or ec 5. A novel, macrophage migration inhibitory factor suicide substrate inhibits motility and growth of lung cancer cells. If a firm is not confident in its ability to collect on its credit sales, it may sell the right to receive payment to the factor at a discount. The association between the migration inhibitory factor. This consists of culturing human peripheral blood lymphocytes in tissue culture medium plus antigen, concentrating the supernatant fluids x10, adding ampicillin, glutamine, normal guinea pig sera andor fetal calf sera.
Pdf macrophage migration inhibitory factor in protozoan. This cytokine appears to be a critical regulator of the inflammatory pathways, leading to systemic inflammatory response syndrome and subsequent multiple organ dysfunction syndrome. Upon an inflammatory stimulus, bioactive mif is rapidly released from intracellular storage pools 21. The role of macrophage migration inhibitory factor mif and autophagy in gastric cancer is not clear. We then used linear discriminant analysis to calculate. Macrophage migration inhibitory factor mif is an important regulator of the host response to infection. Pdf macrophage migration inhibitory factor mif is a pluripotent cytokine which plays roles in inflammation, immune responses and cancer development. The protein known as macrophage migration inhibitory factor mif was one of the first cytokines to be discovered. Pdf macrophage migration inhibitory factor mif emerged in recent years. The cytokine macrophage migration inhibitory factor mif regulates innate immunity and has diverse influences on inflammation. Macrophage migration inhibitory factor mif is implicated in the regulation of inflammation and cell growth. Mif expression was evaluated by immunohistochemistry on formalin fixed skin biopsies of patients with dssc and controls. Released by various immune cells and by the anterior pituitary gland, mif plays a critical role in the systemic inflammatory response by counterregulating the inhibitory effect of glucocorticoids on immunecell activation and proinflammatory.
Migration inhibitory factor definition of migration. In the present paper, we discussed the participation of mif in the immune. Targeting macrophage migration inhibitory factor mif has improved the outcomes of certain inflammatory diseases, but its role in influenza viral infection is. Mif production is absent in certain immunodeficiency disorders, such as wiskottaldrich syndrome and hodgkins disease. Serum mif and urinary monocyte chemoattractant protein 1 umcp1 were.
Influenzaassociated mortality continues to occur annually despite available antiviral therapies. Macrophage migration inhibitory factor mif was first identified nearly 50 years ago and has been used as a cytokine and an enzyme. Peripheral blood lymphocytes from dogs sensitized to streptolysin o slo were assayed for migration inhibitory factor mif production by the indirect mif test, using guinea pig peritoneal exudate cells as the source of macrophages. Macrophage migration inhibitory factor mif, also known as glycosylationinhibiting factor gif, ldopachrome isomerase, or phenylpyruvate tautomerase is a protein that in humans is encoded by the mif gene. Mif is now recognized to be a multipotent cytokine involved in the regulation of immune and inflammatory responses. Macrophage migration inhibitory factor mif is a pleiotropic cytokine produced by the pituitary gland and multiple cell types, including macrophages mo, dendritic cells dc and tcells. Objective to characterise the clinical relevance of urinary macrophage migration inhibitory factor umif concentrations in patients with systemic lupus erythematosus sle. A novel allosteric inhibitor of macrophage migration.
Migration inhibitory factor mif is a proinflammatory cytokine that plays. Macrophage migration inhibitory factor mif prevents. The factor then assumes the credit risk associated. Hendgencotta 1, pia stock 1, peter luedike 1, hideo andreas baba 2, markus kamler 3 and tienush rassaf 1 1 department of cardiology and vascular medicine, west german heart and vascular center, university. Analysis of urinary macrophage migration inhibitory factor. Patients diagnosed with cultureconfirmed ptb without treatment were included and the.
Macrophage migration inhibitory factor acts as the potential target. Mif macrophage migration inhibitory factor promoter polymorphisms and susceptibility to severe malarial anemia. Benign lymphoepithelial lesion blel is a rare disease observed in the adult population. Prognostic role of macrophage migration inhibitory factor. Activity and properties of macrophage migration inhibitory factor.
Migration inhibitory factor article about migration. Methods mif, adjusted for urine creatinine, was quantified by elisa in urine samples from 64 prospectively recruited patients with sle. Macrophage responses migration inhibitory factor modulates host. Get a printable copy pdf file of the complete article 850k, or click on a.
This chapter describes a method for the detection of mif. Emerging role of the macrophage migration inhibitory factor. Inhibition of macrophage migration inhibitory factor mif pnas. Myocardial expression of macrophage migration inhibitory. A modified direct macrophage migrationinhibition factor mif test for the different drugs was carried out on blood samples from these patients. Migration inhibitory factor an overview sciencedirect. The production by lymphocytes of this migration inhibitory factor mif is immunologically specific. Elhassan, ahmed mohammed, ishag adam, at fresearch. Here, we characterize the functional link between mif and e2f transcription factors.
Migration inhibitory factor legal definition of migration. Recombinant human migration inhibitory factor has adjuvant activity article pdf available in proceedings of the national academy of sciences 941. Using gel filtration and preparative isotachophoresis, the migration inhibitory factor mif was highly purified from human lymphocytes activated with concanavalin a. Migration inhibitory factor mif is a secretory product of corticotropic and thyrotropic pituitary cells released in response to stress. Macrophage migration inhibitory factor mif 2 is a multifunctional protein that is both a cytokine and an enzyme possessing tautomerase activity. Entamoeba histolyticaencoded homolog of macrophage.
Sixty cc blood is drawn from the donor and delivered into the 2 test tubes which contain heparin and mixed thoroughly at once. Correlation between macrophage migration inhibitory factor. Among these, macrophage migration inhibitory factor mif was observed to be highly overexpressed in two of the invasive cell lines. Here we measured absolute mrna values a reliable quantitation of number of molecules of macrophage migration inhibitory factor and interleukin1. In the capacity of a cytokine, mif functions as a central upstream mediator of innate immunity and a regulator of inflammation. Macrophage migration inhibitory factor mif was originally identified in the culture medium of activated t lymphocytes as a soluble factor that inhibited the random migration of macrophages. Deficient deletion of apoptotic cells by macrophage. The protein known as macrophage migration inhibitory factor mif has emerged as an important regulator of inflammation 7, 8, playing a central role in the control of both innate and antigenspecific immunity 9 14.
Migration inhibitory factor test definition of migration. A specific direct correlation was established between the degree of inhibition of migration and the concentration of slostimulated supernatants from. Migrations have occurred throughout history and hav. To examine whether the expression levels of mif were correlated. Data were compared by the kruskalwallis test and dunn multiple. Macrophage migration inhibitory factor a therapeutic. The cytokine macrophage migration inhibitory factor mif has emerged to be an. A macrophage migration inhibitory factor promoter polymorphism is associated with highdensity parasitemia in children with malaria. Macrophage migration inhibitory factor and placental malaria infection in an area characterized by unstable malaria transmission in central sudan read the latest article version by reem eltayeb, naser bilal, awadelkareem abass, elhassan m. The cytokine macrophage migration inhibitory factor mif was first discovered 40 years ago as a tcellderived factor that inhibited the random migration of macrophages 12. Mif is believed to initiate inflammation by promoting the release of proinflammatory mediators, such. Migration inhibitory factor mif is a proinflammatory cytokine that plays important roles in physiology, pathology, immunology and parasitology, including the control of infection by protozoa parasites such as toxoplasma gondii. Macrophage migration inhibitory factor mif office of.
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